Composite

Part:BBa_K4907141

Designed by: Xinyi Yang   Group: iGEM23_XMU-China   (2023-09-16)


araBAD-weak-B0034-ccdB

Biology

pBAD/araC promoter consists of two structures, namely araC and araBAD promoter. We found that BBa_K206001 and araBAD promoter part overlapped, so we tried to combine BBa_K206001 with the unoverlapped part to build an araBAD-weak promoter.

Usage

We constructed composite part:BBa_K4907141.

Fig. 1: Gene Circuit of BBa_K4907141

Characterization

Colony PCR

In the construction of this circuit, colony PCR and gene sequencing were used to verify the correctness of the transformants. At a position around 916bp, a target band of approximately 1000bp was observed (Fig. 2).

Fig. 2 DNA gel electrophoresis of the colony PCR products of BBa_K4907141_pSB4k5

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 546
    Illegal SapI site found at 56


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Categories
Parameters
None